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While these findings do not establish diagnostic biomarkers, they underscore the relevance of metabolic profiling in ASD and support further investigation into dietary strategies as adjunctive, individualized interventions

However, a ratio approach can complicate interpretation of data, if it is unclear whether the reference neurochemical is altered by treatment as well

To study the permeability of nanoparticles in vitro BBB models, which conform to the 3R principles with the minimalization the number of animal experiments, are widely used either in static [34] or in dynamic conditions by employing lab-on-a-chip systems [35]

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